Nucleic Acids Research, 1980, Vol. 8, No. 6 1231-1242
© 1980
Articles |
Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-
2a heavy chain
Unite de Genie Genetique, ERA C.N.R.S. 201, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
Received February 12, 1980.
A DNA sequence complementary to the entire coding part of a mouse
2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the PstI site of pBR 322. Transformants containing sequences complementary to purified
2a heavy chain mRNA were selected. One transformant, pG2a-1021, containing a 1750 nucleotide insert, has been characterized by hybrid-arrested translation and purification of
2a heavy chain mRNA on DNA-DBM cellulose filters. Restriction enzyme analysis and partial sequencing demonstrate that the pG2a-1021 contains the complete structural sequence for the
2a heavy chain and predicts the sequence of a 18 amino acid hydrophobic amino terminal extra piece segment.
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