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Nucleic Acids Research, 1980, Vol. 8, No. 6 1231-1242
© 1980


Articles

Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-{gamma} 2a heavy chain

C. Auffray, R. Nageotte, B. Chambraud and F. Rougeon

Unite de Genie Genetique, ERA C.N.R.S. 201, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France

Received February 12, 1980. A DNA sequence complementary to the entire coding part of a mouse {gamma}2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the PstI site of pBR 322. Transformants containing sequences complementary to purified {gamma}2a heavy chain mRNA were selected. One transformant, pG2a-10–21, containing a 1750 nucleotide insert, has been characterized by hybrid-arrested translation and purification of {gamma}2a heavy chain mRNA on DNA-DBM cellulose filters. Restriction enzyme analysis and partial sequencing demonstrate that the pG2a-10–21 contains the complete structural sequence for the {gamma}2a heavy chain and predicts the sequence of a 18 amino acid hydrophobic amino terminal extra piece segment.


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