Skip Navigation

This Article
Right arrow Print PDF (470K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Belintsev, B.N.
Right arrow Articles by Shemyakin, M.F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Belintsev, B.N.
Right arrow Articles by Shemyakin, M.F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 6 1391-1404
© 1980

Articles

On the promoter complex formation rate of E.coli RNA polymerases with T7 phage DNA

B.N. Belintsev*, S.K. Zavriev** and M.F. Shemyakin***

*Institute of Molecular Biology, USSR Academy of Sciences Moscow **Institute of Physiology, Georgian SSR Academy of Sciences Tbilisi ***M.M.Shemyakin Institute of Bioorganic Chemistry. USSR Academy of Sciences Moscow, USSR

Received January 9, 1980. Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 109 to 3.107 M–1 sec–1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Shimamoto
One-dimensional Diffusion of Proteins along DNA. ITS BIOLOGICAL AND CHEMICAL SIGNIFICANCE REVEALED BY SINGLE-MOLECULE MEASUREMENTS
J. Biol. Chem., May 28, 1999; 274(22): 15293 - 15296.
[Full Text] [PDF]

Home page
 ScienceHome page
H Kabata, O Kurosawa, I Arai, M Washizu, S. Margarson, R. Glass, and N Shimamoto
Visualization of single molecules of RNA polymerase sliding along DNA
Science, December 3, 1993; 262(5139): 1561 - 1563.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.