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Nucleic Acids Research, 1980, Vol. 8, No. 6 1405-1420
© 1980

Articles

Antibodies against the subunits of E.coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands

Walter Stender

Physiologisch-Chemisches Institut, Universität Würzburg Koellikerstrasse 2, D-8700 Würzburg, GFR

Received January 4, 1980. Antibodies against the isolated subunits {alpha}, ß, and ß' of DNA-dependent RNA polymerase from E. coli have been prepared. They have been used to compare the extent of antibody-binding, as measured by complement fixation, to the isolated subunits and to the intact enzyme, in the absence and presence of ligands, such as inhibitors, nucleotides, nucleosides, oligo- and poly-nucleotides, and DNA of different composition. In many cases the results show a subunit-specific dependence of complement fixation upon the presence of a ligand and suggest a functional topography of the interaction between the subunits {alpha}, ß, and ß' of RNA polymerase and defined nucleotide sequences and small ligands.


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