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Nucleic Acids Research, 1980, Vol. 8, No. 7 1445-1457
© 1980

Articles

Yeast mitochondrial methionine initiator tRNA: characterization and nucleotide sequence

J. Canaday+, G. Dirheimer+,* and R.P. Martin+

+Départment de Biochimie, Institut de Biologie Molécularie et Celluaire du CNRS 15 rue Descartes, 67084 Cedex *Faculté de Pharmacie, Université Louis Pasteur BP no 10, 67048 Strasbourg Cedex, France

Received March 7, 1980. Two methionine tRNAs from yeast mitochondria have been purified. The mitochondrial initiator tRNA has been identified by formylation using a mitochondrial enzymes extract. E.coli transformylase however, does not formylate the yeast mitochondrial initior tRNA. The sequence was determined using both 32P-in vivo labeled mt tRNAfMet. This tRNA, unlike N.crassa mitochondrial tRNAfMet, has two structural features typical of procaryotic initiator tRNAs : (i) it lacks a Watson-Crick base-pair at the end of the acceptor stem and (ii) has a T-{psi}-C-A sequence in loop IV. However, both yeast and N.crassa mitochondrial initiator tRNAs have a U11:A24 base-pair in the D-stem unlike procaryotic initiator tRNAs which have A11:U24. Interestingly, both mitochondrial initiator tRNAs, as well as bean chloroplast tRNAfMet, have only two G:C pairs next to the anticodon loop, unlike any other initiator tRNA whatever its origin. In terms of overall sequence homology, yeast mitochondrial tRNAfMet differs from both procaryotic or eucaryotic initiator tRNAs, showing the highest homology with N. crassa mitochondrial initiator tRNA.


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