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Nucleic Acids Research, 1980, Vol. 8, No. 7 1459-1473
© 1980

Articles

The nucleotide sequence surrounding the replication origin of the cop3 mutant of the bacterio-cinogenic plasmid Clo DF13

A.R. Stuitje, E. Veltkamp, J. Maat+ and H.L. Heyneker*

Dep. Genetics, Biological Lab., Free University De Boelelaan 1087, Amsterdam-Buitenveldert, Netherlands +Dep. Biochemistry and Biophysics, University of California San Francisco, CA 94143, USA *Genentech. Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA

Received February 19, 1980. The nucleotide sequence from about 100 base-pairs downstream to about 600 base pairs upstream the CloDF13 replication origin has been determined. A comparison of this sequence with the corresponding ColE1 origin sequence reveals that:

  • The sequence at the origin of replication is conserved.
  • There are large differences in the nucleotide sequence downstream the replication origin, whereas there is a large homology in the region of about 410 base-pairs upstream the replication origin.
  • This conserved region might code for a largely homologous basic, arginine rich polypeptide of about 45 amino-acids, for both ColE1 and CloDF13.
  • Although there are large differences in the primary structure of the region coding for the 100 nucleotide RNA, the secondary structure of this region seems to be conserved.


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