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Nucleic Acids Research, 1980, Vol. 8, No. 7 1675-1691
© 1980

Articles

Azidopolynucleotides as photoaffinity reagents

I.L. Cartwright{dagger} and D.W. Hutchinson*

Department of Chemistry and Molecular Sciences, University of Warwick Coventry, CV4 7AL, UK

*To whom correspondence should be addressed

Received January 24, 1980.

Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphatea using polynucleotide phosphorylase from Escheriohia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single stranded poly(adenylic,8-azidoadenylic acid) [poly(A, z8A)] has been used as a photoaffinity reagent to explore the subunit topography of RNA polymerase from E.coli.

{dagger}Present address: The Biological Laboratories, Harvard University, Cambridge, Mass, 02138, U.S.A.


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