Skip Navigation

This Article
Right arrow Print PDF (2657K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (54)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Razin, A.
Right arrow Articles by Glaser, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Razin, A.
Right arrow Articles by Glaser, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 8 1783-1792
© 1980

Articles

Studies on the biological role of DNA methylation; IV. Mode of methylation of DNA in E. coli cells*

Aharon Razin, Simcha Urieli, Yaakov Pollack, Yosef Gruenbaum and Gad Glaser

Department of Cellular Biochemistry, The Hebrew University - Hadassah Medical School Jerusalem, Israel

Received February 14, 1980.

Two pairs of restriction enzyme isoschizomers were used to study in vivo methylation of E. coli and extrachromosomal DNA. By use of the restriction enzymes MboI (which cleaves only the unmethylated GATC sequence) and its isoschizomer Sau3A (indifferent to a methylated adenine at this sequence), we found that all the GATC sites in E. coli and in extrachromosomal DNAs are symmetrically methylated on both strands. The calculated number of GATC sites in E. coli DNA can account for all its m6Ade residues. Foreign DNA, like mouse mtDNA, which is not methylated at GATC sites became fully methylated at these sequences when introduced by transfection into E. coli cells. This experiment provides the first evidence for the operation of a de novo methylation mechanism for E. coli methylases not involved in restriction modification. When the two restriction enzyme isoschizomers, EcoRII and ApyI, were used to analyze the methylation pattern of Formula sequences in E. coli C and CCTA174 DNA, it was found that all these sites are methylated. The number of Formula sites in E. coli C DNA does not account for all m5Cyt residues.

*Paper III in this series is Friedman, J., Friedmann, A. and Razin, A. (1977) Nucleic Acids Res. 4, 3483–3496.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
A Razin and A. Riggs
DNA methylation and gene function
Science, November 7, 1980; 210(4470): 604 - 610.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.