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Nucleic Acids Research, 1980, Vol. 8, No. 8 1793-1804
© 1980

Articles

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA

J.P. Liautard*,{dagger} and J.M. Egly+,

*Laboratoire de Biochimie, Centre Paul Lamarque Clinique St Eloi, B.P. 5054, 34033 Montpellier Cedex, France +INSERM U. 184, Institut de Chimie Biologique, Faculté de Médecine 11, rue Humann, 67085 Strasbourg Cedex, France

Correspondence to: J.M. EGLY, INSERH U 184, Institut de Chimie Biologique, Facuité de Médecine, 11 rue Humann, 67085 STRASBOURG cedex

Received February 11, 1980.

We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated although free mRNP washed with 0.5 M KCl can direct polypeptide synthesis. Furthermore, the 0.5 M KCl wash possesses a factor which inhibits the translation of 0.5 M KCl washed free mRNP as well as globin mRNA naked mRNA from plasmocytoma, or Hela cells. We also demonstrated that native free mRNP are able to form a complex with ribosomal subunits in the presence of initiation factors. This indicates that inhibition of translation by the 0.5 M KCl wash occurs either at some point after initiation complex formation or at the elongation step.

{dagger}Laboratoire de Biochimie Moléculaire, Université des Sciences et Techniques, 34060 Montpellier Cedex, France


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