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Nucleic Acids Research, 1980, Vol. 8, No. 9 1975-1986
© 1980

Articles

Structure of a yeast non-initiating methionine-tRNA gene

Judith Olah+ and Horst Feldmann

Institut fur Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München Goethestrasse 33, 8000 München 2, GFR

Received March 17, 1980.

4 to 8 kb Hind III fragments of yeast DNA were cloned into pBR322. One of these clones (pY6m3) containing a single tRNAmet3 gene has been characterized in detail. The DNA sequence of the structural gene is colinear with the tRNA sequence, which means that in this case no intervening sequence is present. The 5'-leader and 3'-trailer sequences have also been determined. The 5'-flanking region can be folded up into possible secondary structures.

+Judith Olah's present address: Dept. Genet. and Cell Biol. Univ. Minn., 250 Biol. Sci. Center, St. Paul, Minn. 55108


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