Nucleic Acids Research, 1980, Vol. 8, No. 9 2009-2021
© 1980
Articles |
Studies of the recognition sequence of 
X174 gene A protein. Cleavage site of X gene A protein in St-1 RFI DNA
1Institute of Molecular Biology, State University of Utrecht Utrecht, Netherlands 2Laboratory for Physiological Chemistry, State University of Utrecht Utrecht, Netherlands 3Department of Molecular Cell Biology, State University of Utrecht Utrecht, Netherlands
Received January 29, 1980.
It is already known that 
X gene A protein converts besides X RFI DNA also the RFI DNAs of the single-stranded bacteriophages G4, St-1, 
3 and K into RFII DNA. We have extended this observation for bacteriophages G14 and U3. Restriction enzyme analysis placed the X gene A protein cleavage site in St-1 RF DNA in the HinfI restriction DNA fragment F10 and in the overlapping HaeIII restriction DNA fragment Z7. The exact position and the nucleotide sequence at the 3'-0H end of the nick were determined by DNA sequence analysis of the single-stranded DNA subfragment of the nicked DNA fragment F10 obtained by gelelectrophoresis in denaturing conditions. A stretch of 85 nucleotdes of St-1 DNA around the position of the X gene A protein cleavage site was established by DNA sequence analysis of the restriction DNA fragment Z7F1. Comparison of this nucleotide sequence with the previously determined nucleotide sequence around the cleavage site of X gene A protein in X174 RF DNA and G4 RF DNA revealed an identical sequence of only 10 nucleotides. The results suggest that the recognition sequence of the X174 gene A protein lies within these 10 nucleotides.