Skip Navigation

This Article
Right arrow Print PDF (275K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Singer, R.
Right arrow Articles by Grunberger, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Singer, R.
Right arrow Articles by Grunberger, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 9 2067-2074
© 1980

Articles

Infectivity and reconstitution of TMV RNA modified with N-acetoxy-2-acetylaminofluorene or benzo[a] pyrene 7,8-dihydrodiol 9,10 oxide

R. Singer*, P. Pulkrabek+, I.B. Weinstein+ and D. Grunberger+

*Department of Molecular Biology and Virus Laboratory, University of California Berkeley, CA 94720 +Division of Environmental Sciences and Cancer Center/Institute of Cancer Research, Columbia University, College of Physicians and Surgeons New York, NY 10032, USA

Received February 12, 1980. TMV RNA was modified by two bulky carcinogens, N-acetoxy-2-acetylaminofluorene (AAAF) and (±)-7ß, 8{alpha}- dihydroxy-9{alpha}, 10{alpha}-epoxy-7,8,9,10-tetrahydrobenzo[{alpha}]pyrene (BPDE), and the effects of such substituents on biological and physical properties was studied. For both types of modification, the loss of infectivity was directly proportional to the number of chemical modifications indicating that all modifications are lethal. Neither AAAF nor BPDE produced measurable mutations. Reconstitution of modified RNA with TMV protein was partially inhibited, but such inhibition occurred to similar extents with either carcinogen and at varying levels of modification. The data suggest that both types of substitution of TMV RNA generally permit the TMV coat protein to aggregate normally around the RNA, but that AAAF and BPDE may induce some conformational change in the initiation region that inhibits the initiation step.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.