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Nucleic Acids Research, 1980, Vol. 8, No. 9 2085-2091
© 1980

Articles

Structure of transfer RNA by carbon NMR: resolution of single carbon resonances from 13C-enriched, purified species

Paul F. Agris and Paul G. Schmidt

Division of Biological Sciences, University of Missouri Columbia, MO 65211, and Oklahoma Medical Research Foundation and Department of Biochemistry and Molecular Biology, Oklahoma University Health Science Center Oklahoma City, OK 73104, USA

Received February 8, 1980. Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguanosine (m7g) and the methylthio group of 2-methylthio-N6-({delta}2-isopentenyl) adenosine (ms216A) were resolved. The T methyl signal of tRNAPhe shifted from 12.3 ppm at 450 in the absence of added Mg2+ to 11.1 ppm at 300 in the presence of 10mM MgCl2. The same change in conditions led to a 0.4 ppm shift of the m7G methyl signal in the opposite direction. The relative ease in obtainment of single carbon resonances of purified tRNA species, and display of the sensitivity of their chemical shifts to changes in local structure, are requisite criteria for 13C-NMR to be a useful technique in probing tRNA conformation and its changes during interaction with proteins and other nucleic acids.


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