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Nucleic Acids Research, 1980, Vol. 8, No. 9 2093-2103
© 1980

Articles

Conformations of 145 base pair length poly (dG-dC)·poly (dG-dC) in solution and in association with histones

Robert T. Simpson* and Heisaburo Shindo+

*Laboratory of Nutrition and Endocrinology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health Bethesda, MD 20205, USA +Laboratory of Chemical Physics, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health Bethesda, MD 20205, USA

Received January 22, 1980. We have studied the conformation of poly(dG-dC)·poly(dG-dC) in three conditions; i) associated with histone octamers, ii) alone at ionic strength 0.1, and iii) in solutions of over 2.5 M NaCl. The circular dichroism spectrum for the polymer bound to histones differs from that for the free polymer; the difference spectrum is similar to those for native and poly(dA-dT)·poly(dA-dT)core particles. Under the first two conditions, the 31P NMR spectrum is symmetric with line widths of 91 and 41 Hz, respectively, at 109.3 MHz. In high salt, two 31p peaks of equal intensity are observed, confirming recent results of Patel et al. (1) and indicating an alternating geometry for the phosphodiester backbone. Using this highly homogeneous DNA, we confirm that the Pohl-Jovin transition (2) is an intramolecular rearrangement, not requiring complete strand separation.


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