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Nucleic Acids Research, 1981, Vol. 9, No. 1 183-188
© 1981

MOLECULAR BIOLOGY

The nucleotide sequence of spinach chloroplast methionine elongator tRNA

R. Pirtle{dagger}, J. Calagan{dagger}, I. Pirtle{dagger}, M. Kashdan{dagger}, H. Vreman* and B. Dudock{dagger}

{dagger}Department of Biochemistry, State University of New York at Stony Brook Stony Brook, NY 11794 *Department of Medicine, Stanford University Palo Alto, CA 94304, USA

Received September 17, 1980. The nucleotide sequence of spinach chloroplast methionine elongator tRNA (sp.chl. tRNAmMet) has been determined. This tRNA is considerably more homologous to E. coli tRNAmMet (67% homology) than to the three known eukaryotic tRNAmMet (50–55% homology). Sp.chl. tRNAmMet, like the eight other chloroplast tRNAs sequenced, contains a methylated GG sequence in the dihydrouridine loop and lacks unusual structural features which have been found in several mitochondrial tRNAs.


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