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Nucleic Acids Research, 1981, Vol. 9, No. 1 19-30
© 1981

MOLECULAR BIOLOGY

Cloning of bovine growth hormone gene and its expression in bacteria

E. Keshet, A. Rosner, Y. Bernstein, M. Gorecki* and H. Aviv

Departments of Virology, Weizmann Institute of Science Rehovot, Israel *Departments of Organic Chemistry, Weizmann Institute of Science Rehovot, Israel

Received October 29, 1980. A hybrid plasmid was constructed containing ß-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the ß-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused ß-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH Unlike ß-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.


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