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Nucleic Acids Research, 1981, Vol. 9, No. 11 2455-2474
© 1981

MOLECULAR BIOLOGY

Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes

Barbara K. Felber*, Susan Gerber-Huber*, Christoph Meier+, Felicity E.B. May*, Bruce Westley*, Rudolf Weber* and Gerhart U. Ryffel*

*Zoologisches Institut Sahlistrasse 8 +Mathematisches Institut, Sidlerstrasse 5, Universität Bern 3012 Bern, Switzerland

Received April 14, 1981. The disappearance of defined restriction fragments of the B1-globin, an albumin and the Al vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity factors. DNase I digestion of chromatin revealed for the same gene fragments sensitivity factors differing over a much wider range. This is correlated to the activity of the genes analyzed: the B1-globin gene fragment is more sensitive to DNase I in chromatin of erythrocytes compared to hepatocytes whereas the albumin gene fragment is more sensitive to DNase I in chromatin of hepatocytes. The Al vitellogenin gene has the same DNase I sensitivity in both cell types. Comparing the DNase I sensitivity of the three genes in their inactive state we suggest that different chromatin conformations may exist for inactive genes.


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