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Nucleic Acids Research, 1981, Vol. 9, No. 12 2675-2689
© 1981

MOLECULAR BIOLOGY

Construction of bacterial plasmids containing sequences complementary to chicken {alpha}-tropomyosin mRNA

A.R. MacLeod

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK

Received May 7, 1981. Recombinant plasmids have been constructed which contain sequences complementary to the mRNA coding for skeletal muscle {alpha}-tropomyosin. These recombinants were detected initially using a selective cDNA probe and subsequently using a messenger RNA selection assay. {alpha}-TM plasmids hybridize to a single mRNA species smaller than 18S ribosomal RNA and found only in skeletal muscle. Cross-hybridization with mRNA's coding for other tropomyosins could not be detected under normal conditions. However, under conditions of reduced stringency {alpha}-TM plasmids cross - hybridize with an RNA species in heart muscle which may code for cardiac tropomyosin.


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