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Nucleic Acids Research, 1981, Vol. 9, No. 12 2933-2947
© 1981

MOLECULAR BIOLOGY

Hemimethylated duplex DNAs prepared from 5-azacytidine-treated cells

Peter A. Jones and Shirley M. Taylor

Division of Hematology-Oncology, Departments of Pediatrics and Biochemistry, Childrens Hospital of Los Angeles, USC School of Medicine 4650 Sunset Boulevard, Los Angeles, CA 90027, USA

Received March 16, 1981. Duplex heavy-light (HL) DNAs synthesized in the presence of BrdUrd and methylation inhibitors were separated from bulk cellular DNA by CsCl density gradient centrifugation and analysed for 5-methylcytosine (5mC) contents by HPLC. DNAs synthesized in the presence of 5 mM ethionine or 2 mg/ml cycloleucine were not detectably hypomethylated, whereas the DNA synthesized in the presence of 2-10µM 5-azacytidine was undermethylated with respect to control DNA. The heavy, or H-strand, in which up to 5% of the cytosine residues were replaced by intact 5-azacytosine, was undermethylated and the HL duplex DNA was therefore strand asymmetrically methylated. This duplex DNA served as an efficient substrate for a crude DNA methyltransferase preparation which transferred the methyl group from S-adenosylmethionine specifically into cytosine residues within the hypomethylated H strand. Increasing levels of incorporated 5-azacytosine inhibited the action of the methyltransferase suggesting that incorporation of 5-azacytosine into DNA may be responsible for the inhibitory effect of 5-azacytidine on DNA methylation.


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