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Nucleic Acids Research, 1981, Vol. 9, No. 12 2949-2959
© 1981

MOLECULAR BIOLOGY

Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNAGlu3 gene

Horst Feldmann, Judith Olah+ and Hildegard Friedenreich

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München Goethestrasse 33, 8000 München 2, GFR

+Judith Olah's present address: Dept. Genet, and Cell Biol.Univ. Minn., 250 Biol.Sci.Center, St. Paul, Minn. 55108, USA

Received March 12, 1981. The sequence of a 1.9 kb Bam x Hind III fragment from yeast has been determined. This fragment is part of a yeast 6.7 kb Hind III segment cloned into pBR322 (pY2O). The fragment carries a single gene for a glutamate tRNA which has no intron. According to genetic analyses [l] this fragment also contains a yeast chromosomal replicator. We have analyzed the sequence for potential open reading frames and for several structural features which are thought to be involved in the initiation of DNA replication. Hybridization studies have revealed that portions of this sequence are repeated within the yeast genome.


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