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Nucleic Acids Research, 1981, Vol. 9, No. 13 3061-3075
© 1981

MOLECULAR BIOLOGY

Cloning of a new mouse foetal ß-globin mRNA sequence

Nabeel A.Affara, Peter S.Goldfarb, Keith Vass, Alan Lyons and Paul R.Harrison

Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK

Received April 15, 1981. A novel globin cDNA recombinant (pFG5) has been isolated from a 14-15 day porton mouse foetal liver cDNA library. It codes for a (ß-like globin mRNA expressed in foetal liver-derived erythroblasts and erythrocytes but not in adult reticulocytes nor in yolk sac derived nucleated erythrocytes. It is also found in Friend cells induced to differentiate by DMSO.

The nucleotide sequence of pFG5 confirms that it does not code for the ß major or ß minor globin chains nor the embryonic {varepsilon} Y2 globin chain; but it is identical to the published partial sequence of the {varepsilon} Y3, globin gene over the region of overlap (78 nucleotides).


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