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Nucleic Acids Research, 1981, Vol. 9, No. 13 3129-3138
© 1981

ENZYMOLOGY

Inhibitory effects of 3'-deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells

Mineo Saneyoshi*, Jun'ichi Tohyama, Chikao Nakayama, Shigeharu Takiya and Masaki Iwabuchi+

*Faculty of Pharmaceutical Sciences +Department of Botany, Faculty of Science, Hokkaido University Sapporo 060, Japan

Received April 15, 1981. 3'-Deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate were synthesized starting from cordycepin in good yield.

The inhibitory effects of these nucleotides were examined in comparison with that of cordycepin 5'-triphosphate (3'-dATP) using purified DNA-dependent RNA polymerases I and II from Dictyoatelium discoideum cells. Both nucleotide analogues strongly and competitively inhibited the incorporations of CTP and UTP into RNA by the RNA polymerases. The Km and Ki values for CTP and 3'-dCTP were 6.3 µM and 3.0 µM, respectively, and those for UTP and 3'-dUTP were 6.3 µM and 2.0 µM, respectively. These two analogues will be useful in studies at the molecular level on the relationship of template and substrate in RNA synthesis with chromatin, isolated nuclei or permeable cells, because they do not have any effect on poly (rA) synthesis.


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