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Nucleic Acids Research, 1981, Vol. 9, No. 14 3419-3432
© 1981

MOLECULAR BIOLOGY

The DNA sequence of the promoter-attenuator of the ilvGEDA operon of Salmonella typhimurium

Michael P. Taillon, D. Amanda Gotto and Robert P. Lawther

Department of Biology, University of South Carolina Columbia, SC 29208, USA

Received March 16, 1981. The isolation of a {lambda}gt.ilvGEDA.S.t. hybrid transducing phage has permitted the characterization of the promoter-attenuator region of the ilvGEDA operon of Salmonella typhimurium. In vitro transcription and Southern hybridization indicate that the promoter-attenuator resides on a 400 nucleotide Rsa I restriction fragment. DNA sequence analysis shows only seven base pair differences exist between the DNA sequence of the ilvGEDA promoter-attenuator of S. typhimurium and that previously published for Escherichia coli K12.


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