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Nucleic Acids Research, 1981, Vol. 9, No. 14 3433-3449
© 1981

MOLECULAR BIOLOGY

Abundant androgen regulated mRNAs in mouse submandibular gland: cell-free translation of renin precursor mRNA

Richard E. Pratt1, Victor J. Dzau1 and Andre J. Ouellette2,*

1Hypertension Unit, Brigham & Women's Hospital, and Dep. Medicine, Harvard Medical School Boston, MA 02114, USA 2Cell Biology Unit, Shriners Burns Institute, Massachusetts General Hospital and the Departments of Surgery and Biological Chemistry, Harvard Medical School Boston, MA 02114, USA

Address for correspondence: Andre J.Ouellette, Cell Biology Unit, Shriners Burns Institute, 51 Blossom Street, Boston, MA 02114, USA

Received March 4, 1981. Submandibular glands of male mice contain at least four abundant mRNAs that occur at low concentrations in qlands of females. The male-specific mRNAs code for polypeptides of 48,000, 43,000, 29,000, and 27,000 MW. Androgenic regulation of these mRNAs is illustrated by their apparent absence in glands of castrate males and by their accumulation in glands of females treated with testosterone. Selective hybrid-arrested translation experiments also indicate reduced levels of these male-specific sequences in female gland cytoplasm. The 48,000 MW male-specific polypeptide is reduced in translation products directed by gland mRNA from C57BL10/J mice (variants deficient in salivary renin), suggesting the corresponding mRNA codes for a renin precursor. The identity of this polypeptide is confirmed by immune selection with renin-specific antibody.


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