Skip Navigation

This Article
Right arrow Print PDF (483K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (49)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gopalakrishna, Y.
Right arrow Articles by Sarkar, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gopalakrishna, Y.
Right arrow Articles by Sarkar, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 14 3545-3554
© 1981

MOLECULAR BIOLOGY

Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA isolation procedure

Yanamandra Gopalakrishna, Donna Langley and Nilima Sarkar

Department of Metabolic Regulation, Boston Biomedical Research Institute Boston, MA 02114, USA Department of Biological Chemistry, Harvard Medical School Boston, MA 02115, USA

Received April 20, 1981. A new one-step procedure for the isolation of bacterial RNA, involving lysis by proteinase K in the presence of sodium dodecyl sulfate, is described. Pulse-labeled RNA isolated by this procedure from Bacillus brevis, Bacillus subtilis, and Escherichia coli B has been found to contain a substantial fraction (15–40%) of polyadenylated RNA as determined by adsorption to oligo (dT)-cellulose. This contrasts with RNA isolated by procedures involving phenol extraction, a process which appears to lead to the selective loss of polyadenylated RNA. The presence of polyadenylated RNA in E. coli was confirmed by an independent method which involved hybridization with [3H]polyuridylic acid. Using the proteinase K method for RNA isolation, it was possible to demonstrate the in vitro synthesis of polyadenylated RNA by toluenetreated cells of B. brevis, B. subtilis, and E. coli.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
C. Condon
RNA Processing and Degradation in Bacillus subtilis
Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 157 - 174.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Holmstrom and L. Gram
Elucidation of the Vibrio anguillarum Genetic Response to the Potential Fish Probiont Pseudomonas fluorescens AH2, Using RNA-Arbitrarily Primed PCR
J. Bacteriol., February 1, 2003; 185(3): 831 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Holmstrøm, T. Tolker-Nielsen, and S. Molin
Physiological States of Individual Salmonella typhimurium Cells Monitored by In Situ Reverse Transcription-PCR
J. Bacteriol., March 15, 1999; 181(6): 1733 - 1738.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.