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Nucleic Acids Research, 1981, Vol. 9, No. 15 3779-3791
© 1981

MOLECULAR BIOLOGY

Nonhistone chromatin proteins HMG-14 and HMG-17 bind preferentially to single-stranded DNA

Paul J. Isackson and Gerald R. Reeck

Department of Biochemistry, Kansas State University Manhattan, KS 66506, USA

Received May 27, 1981. Proteins extracted from chicken erythrocyte chromatin with 0.35 M NaCl were subjected to sequential chromatography on columns containing immobilized double-stranded and single-stranded DNA's. Two-dimensional electrophoresis of protein fractions revealed that HMG-14 and HMG-17 are among the proteins that are retained by the single-stranded DNA column in 0.2 M NaCl/1 mM Tris-Cl (pH 7.5) after having failed to be retained by the double-stranded column under the same conditions. That suggests that those two proteins possess preferential affinity for single-stranded DNA. Further evidence for that was provided by chromatography of purified HMG-14 and of purified HMG-17 on singlestranded and double-stranded DNA columns. We discuss the possible relevance of our results to suggested functions of HMG-14 and HMG-17.


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