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Nucleic Acids Research, 1981, Vol. 9, No. 15 3835-3850
© 1981

MOLECULAR BIOLOGY

Usage of the three tennination codons in a single eukaryotic cell, the Xenopus laevis oocyte

M. Bienz1, E. Kubli1, J. Kohli2, S. deHenau3, G. Huez3, G. Marbaix3 and H. Grosjean3

1Zoological Institute, University of Zürich-Irchel Winterthurerstrasse 190, CH-8057 Zürich, Switzerland 2Institute of General Nicrobiology, University of Berne Altenbergrain 21, CH-3013 Bern, Switzerland 3Laboratoires de Chimie Biologique, Université de Bruxelles 67, Rue des Chevaux, B-1640 Bruxelles, Belgium

Received May 5, 1981. Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogeneous mRNAs were then separated on two–dimensional gels. All three termination codons are used in a single cell, the Xenopus laevis oocyte. But a surprisingly low number of readthrough polypeptides were observed from the 500 mRNAs studied in comparison to uninjected oocytes. The experimental data are compared with the conclusions obtained from the compilation of all available termination sequences on eukaryotic and prokaryotic mRNAs. This comparison indicates that the apparent resistance of natural termination codons against readthrough, as observed by the microinjection experiments, cannot be explained by tandem or very close second stop codons. Instead it suggests that specific context sequences around the termination codons may play a role in the efficiency of translation termination.


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