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Nucleic Acids Research, 1981, Vol. 9, No. 16 4061-4079
© 1981

CHEMISTRY

Calorimetric study of the complexes between polyuridylic acid and adenylic nucleotides

I.Ya. Gukovsky, A.S. Gukovskaya, A.G. Sukhomudrenko and B.I. Sukhorukov

Institute of Biological Physics, USSR Academy of Sciences, Pushchino Moscow Region 142292, USSR

Received June 30, 1981. Soluble complexes of poly(U) and adenylic nucleotides in NaCl solutions were studied by scanning microcalorimetry. Themelting enthalpies, {Delta}Hm of poly(U) complexes with adenosine, 2',3'-cAMP, 2'(3')-AMP, 5'-AMP, ADP, ATP in 1 M NaCl are 50.5; 45.0; 42.9; 28.6; 26.1 and 25.6 kJ/mole triplets, respectively. {Delta}Hm is independent of the complex melting temperature, Tm. The calorimetric enthalpies are considerably lower than the apparent {Delta}Hy.H. obtained from Tm dependence on free monomer concentration. The enthalpy of complex formation in 1 M NaCl depends neither on the number nor on the degree of ionization of the phosphate groups but is essentially determined by their 5'– or 2'(3')-position. In contrast to 2'(3')- AMP.2 poly(U), {Delta}Hm of 5'-AMP.2 poly(U) increases considerably at lowering Na+ concentration. The enthalpy of poly(U) double helix melting in 1 M NaCl is 8.8 kJ/mole pairs which is 2.5 times lower than that in MgCl2 solutions.


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