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Nucleic Acids Research, 1981, Vol. 9, No. 16 4189-4199
© 1981

MOLECULAR BIOLOGY

Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1

Roger J. Watson, Kenichi Umene and Lynn W. Enquist

Laboratory of Molecular Virology, National Cancer Institute Bethesda, MD 20205, USA

Received April 22, 1981. We describe the nucleotide sequence of a herpes simplex virus type 1 DNA fragment containing the intron of the immediate-early mRNA-5 (IE mRNA-5) gene. The location of the intron within this fragment was determined by a Berk & Sharp nuclease S1 protection analysis, and by cloning and sequencing cDNA containing sequences overlapping the IE mRNA-5 splice point. We found that the 149 base pair (bp) intron contained four copies of an identical 23 bp GC rich tandem repeat followed by a further reiteration consisting of the first 15 bp only.


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