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Nucleic Acids Research, 1981, Vol. 9, No. 17 4239-4250
© 1981

MOLECULAR BIOLOGY

The conformation of adenovirus VAI-RNA in solution

Hans-Jürg Monstein and Lennart Philipson

Department of Microbiology, The Biomedical Center Box 581, S-751 23 Uppsala, Sweden

Received July 13, 1981. The secondary structure of an adenovirus associated low molecular weight RNA (VAI-RNA) has been studied by partial digestion with T1-RNase and S1-endonuclease followed by T1-fingerprint analysis. The empirical secondary structure has been compared with two computer generated models based on mimimal free energy of the structure. The results suggest that VAI-RNA in solution has a compact structure with a free energy of around -60 kcal with two stems and four bulge regions. The implication of this structure for the function of VAI-RNA is discussed.


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V. K. Coventry and G. L. Conn
Analysis of adenovirus VA RNAI structure and stability using compensatory base pair modifications
Nucleic Acids Res., March 1, 2008; 36(5): 1645 - 1653.
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