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Nucleic Acids Research, 1981, Vol. 9, No. 17 4339-4354
© 1981

MOLECULAR BIOLOGY

Transcription of the ß-like globin genes and pseudogenes of the goat in a cell-free system

D.S. Luse1, J.R. Haynes2, D. VanLeeuwen2, E.A. Schon2, M.L. Cleary2, S.G. Shapiro2, J.B. Lingrel2 and R.G. Roeder1

1Department of Biological Chemistry, Washington University School of Medicine St. Louis, MO 63110 2Department of Biological Chemistry, University of Cincinnati College of Medicine 231 Bethesda Avenue, Cincinnati, OH 45267, USA

Received April 30, 1981. We have examined the transcription in a cell-free system of all the members of a highly regulated gene family, the ß-like hemoglobin genes of the goat. These five genes, which code for embryonic, fetal, juvenile, and adult ß-globin proteins, are all transcribed to roughly the same extent in the in vitro system. In all cases initiation of transcription is accurate. However, two goat ß-globin pseudogenes, as well as several artificially constructed deletion mutants, are not transcribed in vitro. A common feature of the transcriptionally inactive genes is the lack of an AT-rich consensus sequence just upstream of the presumptive initiation site.


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