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Nucleic Acids Research, 1981, Vol. 9, No. 17 4355-4366
© 1981

MOLECULAR BIOLOGY

The nucleotide sequence of the replication origin of plasmid NTP1

J.N. Grindley and D. Nakada

Department of Biochemistry, School of Medicine, University of Pittsburgh Pittsburgh, PA 15261, USA

Received April 27, 1981. The sequence of the DNA of the origin region of NTP1 has been obtained. Analysis of the sequence indicates that: (1) there is great sequence homology in the DNA upstream from the origin in NTP1, Co1E1, CLODF13, PBR345 AND PBR322; (2) only seven base pairs of NTP1 are identical with the sequence downstream from the origin in Co1E1, although some homology exists for 140 bases downstream; (3) two ten base pair direct repeats are present in NTP1 which are also conserved in all four plasmids named above; (4) probably no polypeptide greater than fifteen amino acids in length is encoded by the NTP1 origin region, since no single open reading frame is conserved in all five plasmids.


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