Skip Navigation

This Article
Right arrow Print PDF (3240K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (132)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Royer-Pokora, B.
Right arrow Articles by Haseltine, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Royer-Pokora, B.
Right arrow Articles by Haseltine, W. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 18 4595-4609
© 1981

MOLECULAR BIOLOGY

Use of exonuclease III to determine the site of stable lesions in defined sequences of DNA: the cyclobutane pyrimidine dimer and cis and trans dichlorodiammine platinum II examples

Brigitte Royer-Pokora, Lynn K. Gordon and William A. Haseltine

Sidney Farber Cancer Institute, Department of Pathology, Harvard Medical School 44 Binney Street, Boston, MA 02115, USA

Received April 20, 1981. A method to detect chemically stable lesions in DNA has been developed using Exonuclease III, a double strand specific nuclease, to digest 5'-end labeled DNA. The products, when analyzed on high resolution DNA sequencing gels, reveal the sites of DNA modification. Cyclobutane pyrimidine dimers induced by UV irradiation can be localized by comparison of the fragments produced by Exonuclease III digestion with fragments obtained after digestion of the DNA with UV specific endonuclease. The experiments demonstrate that Exonuclease III stops one base away from the cyclobutane pyrimidine dimers. Similar experiments with cis- and trans-dichlorodiammine-platinum (II) showed that modifications of DNA by these agents also impede Exonuclease III digestion. In general the same stop sites were found for cis-and trans-platinum adducts. They occur at sites of guanine bases. Additional stop sites were found for cis-platinum at sites of adjacent guanine bases. These results are in agreement with the model that cis-platinum forms intrastrand guanine-guanine dimers, whereas trans-platinum does not.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
H. Schurer, K. Lang, J. Schuster, and M. Morl
A universal method to produce in vitro transcripts with homogeneous 3' ends
Nucleic Acids Res., June 15, 2002; 30(12): e56 - e56.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. P. Davies, L. C. Hardman, and V. Murray
The effect of chromatin structure on cisplatin damage in intact human cells
Nucleic Acids Res., August 1, 2000; 28(15): 2954 - 2958.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Lippard
New chemistry of an old molecule: cis-[Pt(NH3)2Cl2]
Science, December 10, 1982; 218(4577): 1075 - 1082.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.