Nucleic Acids Research, 1981, Vol. 9, No. 18 4627-4637
© 1981
MOLECULAR BIOLOGY |
Probing the principles of amino acid selection using the alanyl-tRNA synthetase from Escherichia coli
Department of Chemistry, Imperial College of Science and Technology London SW7 2AY, UK
Received July 17, 1981.
The alanyl-tRNA synthetase from Escherichia coli activates cysteine, 
-aminobutyrate and other non-cognate amino acids that are larger than alanine so slowly that no editing mechanism is required for error correction. Serine, however, is activated sufficiently rapidly that an editing mechanism is required to remove the products of misactivation. The distinction between the nominally isosteric trio of cysteine, -aminobutyrate and serine by the activation site of the enzyme is attributed to the effect of small differences in size on steric repulsion, the C-O bond length being somewhat shorter than either the C-C or C-S and the van der Waals' radius of -0- being smaller than that of -S- or -CH2-. The smaller amino acid glycine is also readily activated and its reaction products rapidly removed by hydrolytic editing.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Oki, K. Sakamoto, T. Kobayashi, H. M. Sasaki, and S. Yokoyama Transplantation of a tyrosine editing domain into a tyrosyl-tRNA synthetase variant enhances its specificity for a tyrosine analog PNAS, September 9, 2008; 105(36): 13298 - 13303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sheoran, G. Sharma, and E. A. First Activation of D-Tyrosine by Bacillus stearothermophilus Tyrosyl-tRNA Synthetase: 1. PRE-STEADY-STATE KINETIC ANALYSIS REVEALS THE MECHANISTIC BASIS FOR THE RECOGNITION OF D-TYROSINE J. Biol. Chem., May 9, 2008; 283(19): 12960 - 12970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Splan, M. E. Ignatov, and K. Musier-Forsyth Transfer RNA Modulates the Editing Mechanism Used by Class II Prolyl-tRNA Synthetase J. Biol. Chem., March 14, 2008; 283(11): 7128 - 7134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. SternJohn, S. Hati, P. G. Siliciano, and K. Musier-Forsyth Restoring species-specific posttransfer editing activity to a synthetase with a defunct editing domain PNAS, February 13, 2007; 104(7): 2127 - 2132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Sasaki, S.-i. Sekine, T. Sengoku, R. Fukunaga, M. Hattori, Y. Utsunomiya, C. Kuroishi, S. Kuramitsu, M. Shirouzu, and S. Yokoyama Structural and mutational studies of the amino acid-editing domain from archaeal/eukaryal phenylalanyl-tRNA synthetase PNAS, October 3, 2006; 103(40): 14744 - 14749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hati, B. Ziervogel, J. SternJohn, F.-C. Wong, M. C. Nagan, A. E. Rosen, P. G. Siliciano, J. W. Chihade, and K. Musier-Forsyth Pre-transfer Editing by Class II Prolyl-tRNA Synthetase: ROLE OF AMINOACYLATION ACTIVE SITE IN "SELECTIVE RELEASE" OF NONCOGNATE AMINO ACIDS J. Biol. Chem., September 22, 2006; 281(38): 27862 - 27872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. An and K. Musier-Forsyth Cys-tRNAPro Editing by Haemophilus influenzae YbaK via a Novel Synthetase{middle dot}YbaK{middle dot}tRNA Ternary Complex J. Biol. Chem., October 14, 2005; 280(41): 34465 - 34472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sokabe, A. Okada, M. Yao, T. Nakashima, and I. Tanaka Molecular basis of alanine discrimination in editing site PNAS, August 16, 2005; 102(33): 11669 - 11674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. An and K. Musier-Forsyth Trans-editing of Cys-tRNAPro by Haemophilus influenzae YbaK Protein J. Biol. Chem., October 8, 2004; 279(41): 42359 - 42362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Korencic, I. Ahel, J. Schelert, M. Sacher, B. Ruan, C. Stathopoulos, P. Blum, M. Ibba, and D. Soll A freestanding proofreading domain is required for protein synthesis quality control in Archaea PNAS, July 13, 2004; 101(28): 10260 - 10265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-C. Wong, P. J. Beuning, C. Silvers, and K. Musier-Forsyth An Isolated Class II Aminoacyl-tRNA Synthetase Insertion Domain Is Functional in Amino Acid Editing J. Biol. Chem., December 26, 2003; 278(52): 52857 - 52864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Beebe, E. Merriman, and P. Schimmel Structure-specific tRNA Determinants for Editing a Mischarged Amino Acid J. Biol. Chem., November 14, 2003; 278(46): 45056 - 45061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Beuning and K. Musier-Forsyth Hydrolytic editing by a class II aminoacyl-tRNA synthetase PNAS, August 1, 2000; 97(16): 8916 - 8920. [Abstract] [Full Text] [PDF]
|
|