Skip Navigation

This Article
Right arrow Print PDF (383K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (43)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nakamura, M.
Right arrow Articles by Takanami, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakamura, M.
Right arrow Articles by Takanami, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 18 4669-4676
© 1981

MOLECULAR BIOLOGY

Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12

Masataka Nakamura, Masao Yamada, Yukinori Hirota, Kazunori Sugimoto*, Atsuhiro Oka* and Mituru Takanami*

National Institute of Genetics Mishima, Shizuoka 411 *Institute for Chemical Research of Kyoto University Uji-shi, Kyoto 611, Japan

Received July 7, 1981. We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
I. Luque, M. L. Riera-Alberola, A. Andujar, and J. A. G. Ochoa de Alda
Intraphylum Diversity and Complex Evolution of Cyanobacterial Aminoacyl-tRNA Synthetases
Mol. Biol. Evol., November 1, 2008; 25(11): 2369 - 2389.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Min, J. T. Pelaschier, D. E. Graham, D. Tumbula-Hansen, and D. Soll
Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation
PNAS, March 5, 2002; 99(5): 2678 - 2683.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K.-I. Yoshida, Y. Fujita, and S. D. Ehrlich
Three Asparagine Synthetase Genes of Bacillus subtilis
J. Bacteriol., October 1, 1999; 181(19): 6081 - 6091.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. D. Becker and D. Kern
Thermus thermophilus: A link in evolution of the tRNA-dependent amino acid amidation pathways
PNAS, October 27, 1998; 95(22): 12832 - 12837.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. K. B. Berlyn
Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map
Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Boehlein, E. S. Walworth, N. G. J. Richards, and S. M. Schuster
Mutagenesis and Chemical Rescue Indicate Residues Involved in beta -Aspartyl-AMP Formation by Escherichia coli Asparagine Synthetase B
J. Biol. Chem., May 9, 1997; 272(19): 12384 - 12392.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.