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Nucleic Acids Research, 1981, Vol. 9, No. 18 4669-4676
© 1981

MOLECULAR BIOLOGY

Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12

Masataka Nakamura, Masao Yamada, Yukinori Hirota, Kazunori Sugimoto*, Atsuhiro Oka* and Mituru Takanami*

National Institute of Genetics Mishima, Shizuoka 411 *Institute for Chemical Research of Kyoto University Uji-shi, Kyoto 611, Japan

Received July 7, 1981. We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.


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