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Nucleic Acids Research, 1981, Vol. 9, No. 18 4735-4743
© 1981

MOLECULAR BIOLOGY

Variant surface glycoproteins of Trypanosoma brucei are synthesised with cleavable hydrophobic sequences at the carboxy and amino termini

John C. Boothroyd*, Cora A. Paynter*, George A.M. Cross*, Andre Bernards+ and Piet Borst+

*Dep. of Immunochemistry, Wellcome Research Laboratories, Langley Court Beckenham, Kent BR3 3BS, UK +Universiteit van Amsterdam, Afdeling Medische Enzymologie, Lab. voor Biochemie, Jan Swammerdam Institut P.O. Box 60,000 1005 GA, Amsterdam, The Netherlands

Received July 25, 1981. cDNAs coding for the amino and carboxy termini of two trypanosome variant surface glycoproteins (VSGs) have been sequenced. The results indicate that VSGs are synthesised with hydrophobic amino-terminal leader and carboxy-terminal tail sequences which are absent from purified mature VSGs.


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