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Nucleic Acids Research, 1981, Vol. 9, No. 19 4813-4822
© 1981

MOLECULAR BIOLOGY

An unusually long non-coding region in rat lens {alpha}-crystallin messenger RNA

Rob J.M. Moorman*, Huub M.W. van der Velden*, Huub J. Dodemont+, Peter M. Andreoli*, Hans Bloemendal+ and John G.G. Schoenmakers*

*Department of Molecular Biology, University of Nijmegen Toernooiveld, 6525 ED Nijmegen +Department of Biochemistry, University of Nijmegen, Geert Grooteplein 21 6525 EZ Nijmegen, The Netherlands

Received August 5, 1981. Most of the mRNA sequence coding for the {alpha}A2 chain of the ocular lens protein {alpha}-crystallin from rat, has been determined by sequencing cloned DNA copies of this mRNA. The 892-base pair cDNA sequence encompasses all but 52 N-terminal amino acids of the {alpha}A2 chain. It lacks the sequence characteristic for the 22 extra amino acids inserted in the {alpha}A2.-like chain, named {alpha}AIns. A stretch of 583 nucleotides, representing more than 50% of the entire mRNA sequence, is located 3' wards of the {alpha}A2 coding sequence. It contains the characteristic AAUAAA signal involved in poly(A)-addition and represents an unexpectedly long non-coding region. Examination of the total cytoplasmic poly(A)RNA of rat lens by filter-hybridization and subsequent translation of the electrophoretically separated mRNA fractions shows that the {alpha}A2 chain is encoded by mRNA species which are distinct from the {alpha}AIns-encoding mRNA. No evidence is obtained for an extensive size heterogeneity in the 3' untranslated regions of these two different rat lens mRNAs.


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C. King, T Shinohara, and J Piatigorsky
alpha A-crystallin messenger RNA of the mouse lens: more noncoding than coding sequences
Science, February 19, 1982; 215(4535): 985 - 987.
[Abstract] [PDF]


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