Skip Navigation

This Article
Right arrow Print PDF (3308K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (59)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pincus, S.
Right arrow Articles by Rekosh, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pincus, S.
Right arrow Articles by Rekosh, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1981, Vol. 9, No. 19 4919-4938
© 1981

MOLECULAR BIOLOGY

Characterization of the effect of aphidicolin on adenovirus DNA replication: evidence in support of a protein primer model of initiation

Steven Pincus, Warren Robertson and David Rekosh*

Department of Biochemistry, State University of New York at Buffalo Buffalo, NY 14214, USA

Received July 14, 1981.

Adenovirus DNA replication is inhibited by aphidicolin but the inhibition clearly has different parameters than the inhibition of purified DNA polymerase {alpha}. In adenovirus infected Hela cells, 10 µg/ml of aphidicolin reduced viral DNA synthesis by 80%. Cellular DNA synthesis was inhibited by 97% at 0.1 µg/ml. 10 µg/ml of drug had no effect on virus yield or late protein synthesis though higher concentrations of drug (50 ug/ml) caused an abrupt cessation of late protein synthesis and 100 µg/ml reduced virus yield by 3 logs. Concentrations of the drug from 0.5 µg/ml to 10 µg/ml were found to dramatically slow the rate of DNA chain elongation in vitro but not stop it completely, so that over a long period of time net incorporation was reduced only slightly compared to the control. 50 µg/ml or 100 µg/ml of drug completely inhibited incorporation in vitro.

Initiation of viral DNA repTTcafion - covalent attachment of dCMP to the preterminal protein - occurs in vitro. This reaction was found to be insensitive to inhibition by aphidicblin. We thus conclude that aphidicolin exerts its effect on adenovirus DNA chain elongation, but not on the primary initiation event of protein priming.

*To whom reprint requests should dbe addressed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
D. Hoffmann and O. Wildner
Restriction of adenoviral replication to the transcriptional intersection of two different promoters for colorectal and pancreatic cancer treatment.
Mol. Cancer Ther., February 1, 2006; 5(2): 374 - 381.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.