Nucleic Acids Research, 1981, Vol. 9, No. 19 4951-4966
© 1981
MOLECULAR BIOLOGY |
Multiple ribosomal RNA cleavage pathways in mammalian cells
Department of Microbiology and Immunology, Washington University School of Medicine St. Louis, MO 63100, USA
Received July 6, 1981. The sequence content of mouse L cell pre-rRNA was examined by RNA gel transfer and blot hybridization. Nuclear RNAs were separated by agarose gel electrophoresis, transferred to diazo-paper, and hybridized to twelve different restriction fragments that are complementary to various sections of 45S pre-rRNA. An abundant new 34S pre-rRNA and less abundant new 37S, 26S and 17S pre-rRNAs were detected. The presence of these new pre-rRNAs suggests the existence of at least two new pre-rRNA cleavage pathways. 34S and 26S pre-rRNAs were also detected in HeLa cells suggesting that these new cleavage pathways are characteristic of mammalian cells. Further, an abundant new 12S precursor to 5.8S rRNA was also detected and is common to all the proposed cleavage pathways. The previously identified 45S, 41S, 32S and 20S pre-rRNAs were readily detected and their general structure confirmed. The 20S pre-rRNA is characteristic of the known pathway used by HeLa and other cells, and its presence suggests that growing mouse L cells use this pre-rRNA cleavage pathway. The 36S pre-rRNA characteristic of the previously described mouse L cell cleavage pathway was not detected. In all these cleavage pathways pre-rRNA cleavage sites are apparently identical and occur at or near the termini of the mature 18S, 5.8S and 28S rRNA sequences. The pathways differ only in the temporal order of cleavage at these sites.
The position of the 5.8S rRNA sequence was located within the internal transcribed spacer. The known and conserved sequence of 5.8S rRNA from several organisms predicts a characteristic pattern of restriction enzyme sites for 5.8S rDNA. Internal transcribed spacer rDNA was mapped with restriction enzymes, and the characteristic pattern was found near the midpoint of the internal transcribed spacer. This places the 5.8S rRNA sequence at or near the 5' terminus of 32S pre-rRNA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Chen, E. Knutson, S. Wang, L. A. Martinez, and T. Albrecht Stabilization of p53 in Human Cytomegalovirus-initiated Cells Is Associated with Sequestration of HDM2 and Decreased p53 Ubiquitination J. Biol. Chem., October 5, 2007; 282(40): 29284 - 29295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Adachi, C. Soeta-Saneyoshi, H. Sagara, and Y. Iwakura Crucial Role of Bysl in Mammalian Preimplantation Development as an Integral Factor for 40S Ribosome Biogenesis Mol. Cell. Biol., March 15, 2007; 27(6): 2202 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schilders, R. Raijmakers, J. M. H. Raats, and G. J. M. Pruijn MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation Nucleic Acids Res., December 7, 2005; 33(21): 6795 - 6804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Supowit, R. T. Ethridge, H. Zhao, K. A. Katki, and D. J. DiPette Calcitonin gene-related peptide and substance P contribute to reduced blood pressure in sympathectomized rats Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1169 - H1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Hefferan, G. L. Evans, S. Lotinun, M. Zhang, E. Morey-Holton, and R. T. Turner Effect of gender on bone turnover in adult rats during simulated weightlessness J Appl Physiol, November 1, 2003; 95(5): 1775 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Geiger, J. P. Bailey, W.-Z. Zhan, C. B. Mantilla, and G. C. Sieck Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle J Appl Physiol, August 1, 2003; 95(2): 611 - 619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Strezoska, D. G. Pestov, and L. F. Lau Functional Inactivation of the Mouse Nucleolar Protein Bop1 Inhibits Multiple Steps in Pre-rRNA Processing and Blocks Cell Cycle Progression J. Biol. Chem., August 9, 2002; 277(33): 29617 - 29625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Supowit, H. Zhao, D. H. Wang, and D. J. DiPette Omapatrilat in Subtotal Nephrectomy-Salt Hypertension: Role of Calcitonin Gene-Related Peptide Hypertension, September 1, 2001; 38(3): 697 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Chen, E. Knutson, A. Kurosky, and T. Albrecht Degradation of p21cip1 in Cells Productively Infected with Human Cytomegalovirus J. Virol., April 15, 2001; 75(8): 3613 - 3625. [Abstract] [Full Text]
|
|
|
|
|
|
S. C. Supowit, H. Zhao, and D. J. DiPette Nerve Growth Factor Enhances Calcitonin Gene-Related Peptide Expression in the Spontaneously Hypertensive Rat Hypertension, February 1, 2001; 37(2): 728 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Strezoska, D. G. Pestov, and L. F. Lau Bop1 Is a Mouse WD40 Repeat Nucleolar Protein Involved in 28S and 5.8S rRNA Processing and 60S Ribosome Biogenesis Mol. Cell. Biol., August 1, 2000; 20(15): 5516 - 5528. [Abstract] [Full Text]
|
|
|
|
|
|
P. R. R. Gangula, H. Zhao, S. C. Supowit, S. J. Wimalawansa, D. J. Dipette, K. N. Westlund, R. F. Gagel, and C. Yallampalli Increased Blood Pressure in {alpha}-Calcitonin Gene-Related Peptide/Calcitonin Gene Knockout Mice Hypertension, January 1, 2000; 35(1): 470 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-M. Lee, V. Udupi, E. W. Englander, S. Rajaraman, R. J. Coffey Jr., and G. H. Greeley Jr. Stimulatory Actions of Insulin-Like Growth Factor-I and Transforming Growth Factor-{alpha} on Intestinal Neurotensin and Peptide YY Endocrinology, September 1, 1999; 140(9): 4065 - 4069. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Liu, M. Peng, A. M. Laties, and R. Wen Preconditioning with Bright Light Evokes a Protective Response against Light Damage in the Rat Retina J. Neurosci., February 15, 1998; 18(4): 1337 - 1344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Westerlind, J. D. Fluckey, S. E. Gordon, W. J. Kraemer, P. A. Farrell, and R. T. Turner Effect of resistance exercise training on cortical and cancellous bone in mature male rats J Appl Physiol, February 1, 1998; 84(2): 459 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Supowit, H. Zhao, D. M. Hallman, and D. J. DiPette Calcitonin Gene-Related Peptide Is a Depressor in Subtotal Nephrectomy Hypertension Hypertension, January 1, 1998; 31(1): 391 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Supowit, H. Zhao, D. M. Hallman, and D. J. DiPette Calcitonin Gene–Related Peptide Is a Depressor of Deoxycorticosterone-Salt Hypertension in the Rat Hypertension, April 1, 1997; 29(4): 945 - 950. [Abstract] [Full Text]
|
|
|
|
|
|
P. R. R. Gangula, S. C. Supowit, S. J. Wimalawansa, H. Zhao, D. M. Hallman, D. J. DiPette, and C. Yallampalli Calcitonin Gene-Related Peptide Is a Depressor in NG-Nitro-L-Arginine Methyl Ester-Induced Hypertension During Pregnancy Hypertension, January 1, 1997; 29(1): 248 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wen, T. Cheng, Y. Li, W. Cao, and R. H. Steinberg alpha 2-Adrenergic Agonists Induce Basic Fibroblast Growth Factor Expression in Photoreceptors In Vivo and Ameliorate Light Damage J. Neurosci., October 1, 1996; 16(19): 5986 - 5992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Supowit, H. Zhao, D. H. Wang, and D. J. DiPette Regulation of Neuronal Calcitonin Gene–Related Peptide Expression : Role of Increased Blood Pressure Hypertension, December 1, 1995; 26(6): 1177 - 1180. [Abstract] [Full Text]
|
|
|
|
|
|
S. C. Supowit, A. Gururaj, C. V. Ramana, K. N. Westlund, and D. J. DiPette Enhanced Neuronal Expression of Calcitonin Gene–Related Peptide in Mineralocorticoid-Salt Hypertension Hypertension, June 1, 1995; 25(6): 1333 - 1338. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Olswang, H. Cohen, O. Papo, H. Cassuto, C. M. Croniger, P. Hakimi, S. M. Tilghman, R. W. Hanson, and L. Reshef A mutation in the peroxisome proliferator-activated receptor gamma -binding site in the gene for the cytosolic form of phosphoenolpyruvate carboxykinase reduces adipose tissue size and fat content in mice PNAS, January 22, 2002; 99(2): 625 - 630. [Abstract] [Full Text] [PDF]
|
|