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Nucleic Acids Research, 1981, Vol. 9, No. 2 237-246
© 1981

MOLECULAR BIOLOGY

The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34)

Greg Winter, Stan Fields, Michael J. Gait and George G. Brownlee

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK The Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford OX1 3RE, UK

Received November 21, 1980. Complete double-stranded DNA copies of the RNA genes of the human influenza virus A/PR/8/34 have been synthesized by using two synthetic oligodeoxynucleotide primers. The gene encoding the non-structural proteins NS1 and NS2, prepared with these primers, has been cloned into the bacteriophage M13mp7 and sequenced. The sequence is compared with that from another human strain and from an avian strain.


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