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Nucleic Acids Research, 1981, Vol. 9, No. 2 277-292
© 1981

MOLECULAR BIOLOGY

Cyclic AMP receptor protein interacts with lactose operator DNA

A. Schmitz

Département de Biologie Moléculaire, Université de Genève CH-1211 Genève 4, Switzerland

Received November 10, 1980. The wildtype lac promoter-operator region of E.coli was reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the "footprinting’ method. Two distinct interaction sites were found, one in the –60 base pair region, which has previously been described, and a second one covering the lac operator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.


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