Nucleic Acids Research, 1981, Vol. 9, No. 2 309-321
© 1981
MOLECULAR BIOLOGY |
A system for shotgun DNA sequencing
+Department of Bacteriology UC Davis, CA 95616, USA *Division of Organic Chemistry of Genentech, Inc. South San Francisco, CA 94080, USA **Division of Molecular Biology of Genentech, Inc. South San Francisco, CA 94080, USA
Received October 30, 1980.
A multipurpose cloning site has been introduced into the gene for ß-galactosidase (ß-D-galactosidegalactohydrolase, EC 3.21.23) on the single-stranded DNA phage M13mp2 (Gronenborn, B. and Messing, J., (1978) Nature 272, 375–377) with the use of synthetic DNA. The site contributes 14 additional codons and does not affect the ability of the lac gene product to undergo intracistronic complementation. Two restriction endonuclease cleavage sites in the viral gene II ware removed by single base-pair mutations. Using the new phage M13mp7, DNA fragments generated by cleavage with a variety of different restriction endonucleases can be cloned directly. The nucleotide sequences of the cloned DNAs can be determined rapidly by DNA synthesis using chain terminators and a synthetic oligonucleotide primer complementary to 15 bases preceeding the new array of restriction sites.
Present address: Department of Biochemistry, University of Minnesota, St Paul, MN 55108, USA
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|
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|
|
|
|
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|
|
|
|
|
|
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|
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
C Ho, M Jasin, and P Schimmel Amino acid replacements that compensate for a large polypeptide deletion in an enzyme Science, July 26, 1985; 229(4711): 389 - 393. [Abstract] [PDF]
|
|
|
|
|
|
A. Jaiswal, F. Gonzalez, and D. Nebert Human dioxin-inducible cytochrome P1-450: complementary DNA and amino acid sequence Science, April 5, 1985; 228(4695): 80 - 83. [Abstract] [PDF]
|
|
|
|
|
|
T Webster, H Tsai, M Kula, G. Mackie, and P Schimmel Specific sequence homology and three-dimensional structure of an aminoacyl transfer RNA synthetase Science, December 14, 1984; 226(4680): 1315 - 1317. [Abstract] [PDF]
|
|
|
|
|
|
A Wang, S. Lu, and D. Mark Site-specific mutagenesis of the human interleukin-2 gene: structure-function analysis of the cysteine residues Science, June 29, 1984; 224(4656): 1431 - 1433. [Abstract] [PDF]
|
|
|
|
|
|
A. Fornace Jr, D. Cummings, C. Comeau, J. Kant, and G. Crabtree Single-copy inverted repeats associated with regional genetic duplications in gamma fibrinogen and immunoglobulin genes Science, April 13, 1984; 224(4645): 161 - 164. [Abstract] [PDF]
|
|
|
|
|
|
P. Berman, D Dowbenko, L. Lasky, and C. Simonsen Detection of antibodies to herpes simplex virus with a continuous cell line expressing cloned glycoprotein D Science, November 4, 1983; 222(4623): 524 - 527. [Abstract] [PDF]
|
|
|
|
|
|
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|
|
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|
|
|
|
|
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