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Nucleic Acids Research, 1981, Vol. 9, No. 20 5459-5467
© 1981

CHEMISTRY

Z-DNA conformation of N-2-acetylaminofluorene modified poly(dG-dC)·poly(dG-dC) determined by reactivity with anti cytidine antibodies and minimized potential energy calculations

Regina M. Santella, Dezider Grunberger, Suse Broyde1 and Brian E. Hingerty2

Cancer Center/Institute of Cancer Research, Department of Biochemistry and Public Health Division of Environmental Sciences, Columbia University College of Physicians and Surgeons New York, NY 10032, USA

Received July 20, 1981.

The conformation of poly(dG-dC)·poly(dG-dC), poly(dG)·poly(dC), and calf thymus DNA modified with N-acetoxy-N-2-acetylaminofluorene (N-acetoxy-AAF) was examined by extent of reaction with anti cytidine antibodies. In contrast to modified poly(dG)·poly(dC) and DNA, modified poly(dG-dC)·poly(dG-dC) failed to react with the antibodies indicating that the base pairing in this polymer is intact. This is consistent with induction of the Z-DNA conformation in AAF modified poly(dG-dC)·poly(dG-dC). Using minimized potential energy calculations on the dCpdG-AAF dimer as a model for the modified polymer, it is shown that the proposed Z-DNA conformation is energetically stable. A model is proposed for an AAF modified tetramer, dGpdCpdGpdC, in which the AAF is external to the Z-DNA duplex.

1Biology Department, New York University, New York, NY 10003, USA.

2Biology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.


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