Nucleic Acids Research, 1981, Vol. 9, No. 20 5493-5504
© 1981
MOLECULAR BIOLOGY |
Studies on the selectivity of DNA precipitation by spermine
Department of Biochemistry and Molecular Biology, Conant Laboratories, Harvard University Cambridge, MA 02138, USA
Received May 26, 1981.
We have examined the selectivity of the precipitation of DNA by spermine. We have found that the intra- and intermolecular condensation of DNA induced by spermine is highly selective even in the presence of added protein or triphosphates. We have also investigated the influence of buffer components on the threshold concentration of spermine required for DNA precipitation. Representative applications exploiting the selectivity of the precipitation reaction are also described.
*Present address: Department of Biological Sciences, Carnegie-Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
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