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Nucleic Acids Research, 1981, Vol. 9, No. 21 5507-5520
© 1981

MOLECULAR BIOLOGY

Nucleotide sequence of the nbosomal RNA gene of Physarum polycephalum: intron 2 and its flanking regions of the 26S rRNA gene

Hisayuki Nomiyama1, Satoru Kuhara2, Toshio Kukita2,*, Takeshi Otsuka1 and Yoshiyuki Sakaki1,{dagger}

1Department of Biochemistry, Kyushu University 60 School of Medicine Maidashi 2Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University 46-02 Hakozaki, Higashi-Ku, Fukuoka 812, Japan

Received September 23, 1981.

The 26S ribosomal RNA gene of Physarum polycephalum is interrupted by two introns, and we have previously determined the sequence of one of them (intron 1) (Nomiyama et al. Proc.Natl.Acad.Sci.USA 78, 1376–1380, 1981). In this study we sequenced the second intron (intron 2) of about 0.5 kb length and its flanking regions, and found that one nucleotide at each junction is identical in intron 1 and intron 2, though the junction regions share no other sequence homology. Comparison of the flanking exon sequences to E. coli 23S rRNA sequences shows that conserved sequences are interspersed with tracts having little homology. In particular, the region encompassing the intron 2 interruption site is highly conserved. The E. coli ribosomal protein Ll binding region is also conserved.

* Present address: Department of Oral Anatomy, Faculty of Dentistry, Kyushu University, Maidashi, Higashi-Ku, Fukuoka 812.

+ Present address: Research Laboratory for Genetic Information, Kyushu University, Maidashi, Higashi-Ku, Fukuoka 812, Japan.


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