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Nucleic Acids Research, 1981, Vol. 9, No. 21 5671-5678
© 1981

MOLECULAR BIOLOGY

The nudeotide sequence of the promoter and the ammo-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli

Yasuhiro Kikuchi, Koji Yoda, Makari Yamasaki and Gakuzo Tamura

Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Received September 21, 1981. The promoter and the amino-termianl region of phoAM, the structural gene for alkaline phosphatase of Escherichia coli K12, was cloned by using a promoter cloning vector pMC1403. The nudeotide sequence of the cloned fragment has been determined. A sequence encoding the amino-terminal portion of mature alkaline phosphatase is found and it is preceded by a sequence encoding the signal peptide. The signal peptide consists of 21 amino acids; Met-Lys-Gln-Ser-Thr-Ile-Ala-Leu-Ala-Leu-Leu-Pro-Leu-Leu-Phe-Thr-Pro-Val-Thr-Lys-Ala. The translation initiation codon is GUG, which is preceded by the Shine- Dalgarno sequence GGAG. Upstream to these sequences, there is a typical procaryotic promoter, TATAGTC for the Pribnow box. Around the Pribnow box, there are several dyad symmetrical sequences, which may probably be concerned with the regulation of this gene.


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