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Nucleic Acids Research, 1981, Vol. 9, No. 21 5747-5761
© 1981

CHEMISTRY

DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp)

Z.A. Shabarova, N.G. Dolinnaya, V.L. Drutsa, N.P. Mehiikova and A.A. Purmal

Department of Chemistry, and A.N.Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University Moscow 117234, USSR

Received August 13, 1981. Self-association of a decanucleotide d(TGGCCAAGCTp) in an aqueous solution is shown by UV spectroscopy, CD and sedimentation analysis to yield a pseudopolymeric (concatemeric) duplex having a geometry similar to that of DNA B-type. It is demonstrated that in conditions when the concatemeric duplex is stable a water-soluble carbodiimide induces efficient polymerization of the 3'-or 5'-phosphorylated decanucleotide, and the resulting polymers d(TGGCCAAGCTp)2-10 contain only natural phosphodiester bonds. In conditions optimal for template-guided polymerization of d(TGGCCAAGCTp) the overall yield of 20-100-member polynucleotides exceeds 90%. The obtained polymeric duplexes are cleaved by restriction endonuclease Alu I, Bsu RI, and Hind HI to corresponding decamers which were isolated and sequenced.


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