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Nucleic Acids Research, 1981, Vol. 9, No. 21 5763-5784
© 1981

MOLECULAR BIOLOGY

The structural organization of dinudeosomes and oligonucleosomes. Electric dichroism and birefringence study

Claude Houssier, Ignace Lasters, Serge Muyldermans and Lode Wyns

Laboratoire de Chimie Physique, Université de liège (B6) Sart Tilman, B-4000 Liege, Belgium Laboratorium Algemene Biologie, Instituut voor Molekulaire Biologie, Vrije Universiteit Brussels 65, Paardenstraat, B-1640 St-Genesius-Rode, Belgium

Received August 5, 1981. The spatial organization of nucleosomes and linker DNA in dinudeosomes and oligonucleosomes of various chain lengths has been investigated through electric dichroism, birefringence and relaxation times measurements at low ionic strengths (0.5 to 2.2 mM).

From the negative dichroism observed for all the samples, it is concluded that the nucleosome subunits in the oligonucleosome chain must lie with their disc planes closely parallel to the fibre axis.

The large increase of the negative dichroism of dinudeosomes upon Hl removal is interpreted by the unwinding of the DNA tails and internucleosomal segment.

All the samples displayed, under bipolar pulses, a predominantly induced orientation mechanism.


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