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Nucleic Acids Research, 1981, Vol. 9, No. 21 5797-5810
© 1981

ENZYMOLOGY

Purification and characterization of a uracil-DNA glycosylase from the yeast, Saccharomyces cerevisiae

Bill Crosby{dagger}, Louise Prakash{dagger}, Howard Davis+ and David C. Hinkle+

{dagger}Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry Rochester, NY 14642 +Department of Biology, University of Rochester NY 14627, USA

Received August 17, 1981. An activity which releases free uracil from bacteriophage PBS1 DNA has been purified over 10,000 fold from extracts of Saccharornyces cerevisiae. The enzyme is active on both native and denatured PBSl UNA and is active in the absence of divalent cation, and in the presence of 1 mM EDTA. The enzyme has a native molecular weight of 27,800 as estimated by glycerol gradient centrifugation and gelfiltration. Enzyme activity has been recovered after denaturation in SDS and electrophoresis in an SDS polyacrylamide gel. This analysis suggests that the enzyme consists of a single polypeptide chain of about 27,000 daltons. Normal levels of uracil-DNA glycosylase activity were found in partially purified extracts of the nitrous-acid sensitive rad18-2 mutant of yeast.


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