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Nucleic Acids Research, 1981, Vol. 9, No. 21 5845-5854
© 1981

MOLECULAR BIOLOGY

A minimal mechanism for abortive initiation of transcription of T7 DNA

W. Smagowicz and K.H. Scheit

Institute of Biochemistry and Biophysics, Polish Academy of Sciences Rakowiecka 36, Warsaw, Poland Max-Planck-Institut für Biophysikalische Chemie, Abteilung Molekulare Biologie Göttingen, Am Fassberg, GFR

Received June 8, 1981. A steady state kinetic analysis of product inhibition in abortive initiation of transcription at promoters A1 and A3 of T7 DNA was carried out. The obtained kinetic data are in agreement with a mechanism of ordered product release with pppApU being released first from the central complex. A minimal general mechanism for abortive initiation satisfying the kinetic results is given. The stimulation of transcription of T7 DNA by pppApU at low substrate concentrations was investigated.


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