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Nucleic Acids Research, 1981, Vol. 9, No. 22 5979-5990
© 1981

MOLECULAR BIOLOGY

Nucleotide sequence of a Bacillus subtilis promoter recognized by Bacillus subtilis RNA polymerase containing {sigma}37

Charles P. Moran, Jr., Naomi Lang and Richard Losick

The Biological Laboratories, Harvard University Cambridge, MA 02138, USA

Received July 21, 1981. We report the nucleotide sequence of the promoter for a Bacillus subtilis gene (spoVC) whose transcription is controlled by a 37,000 dalton species of B. subtilis sigma factor known as o37 but not by the principal sigma factor of 55,000 daltons ( O55 ). using SI nuclease mapping we show that the startpoint for o37-directed transcription of the spoVC gene in vitro corresponded closely to the 5' terminus of in vivo synthesized spoVC RNA. The binding site for o37 -containing ERA polymerase extended from 43 bp to 51 bp (positions –43 to –51) upstream from the transcription startpoint to 22 bp (position +22) downstream from the startpoint. The nucleotide sequence of the spoVC promoter differed significantly from promoters whose recognition is controlled by {sigma}55 but was similar to other {sigma}37 controlled promoters in regions known to be important in promoter recognition. Our results are consistent with the hypothesis (Lee and Pero, J. Mol. Biol., in press) that sigma factors work by contacting specific bases in both the –35 and –10 regions of promoters.


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